| 1. | Standard guide for detection of nucleic acid sequences by the polymerase chain reaction technique 用多聚酶链式反应技术对核酸序列的测定的标准指南 |
| 2. | Standard guide for detection of nucleic acid sequences of the human immunodeficiency virus hiv - 1 by the polymerase chain reaction technique 用聚合酶链反应技术探测人体免疫缺乏病毒hiv - 1的核酸类的标准指南 |
| 3. | Blast search was used to characterize the sequence feature . the nucleic acid sequence of dseno gene had high similarity to that of other species 以载体pcambia2301为骨架引入pbnzi中的名刘基因,构建了简便、实用的中间载体pca州田ia230lg 。 |
| 4. | Medical microbiology - serodiagnosis of infectious diseases - part 61 : polymerase chain reaction ; special requirements for the detection of nucleic acid sequences of the human immunodeficiency viruses hiv - 1 and hiv - 2 医用微生物学.传染病血清诊断.第61部分:聚合酶链锁反 |
| 5. | Medical microbiology - diagnosis of infectious diseases in serology and molecular biology - part 62 : polymerase chain reaction ; special requirements for the detection of nucleic acid sequences of the mycobacterium tuberculosis complex 医用微生物学.传染病的血清学和分子生物学诊断.第62 |
| 6. | Research to date suggests that sense and antisense peptides coded for by mutually complementary nucleic acid sequences are capable of interacting specifically , thereby suggesting the existence of a second , two - dimensional genetic code ( i . e . , proteomic code ) 研究表明由互补的核酸序列编码的有义肽和反义肽之间存在特殊的相互作用,由此就提出了有第二套二维的遗传密码即蛋白密码的存在。 |
| 7. | Nucleic acid sequences of azoreduclase were searched and blasted in genbank . a pair of primers based on the conserved regions were designed . a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced . the sequence contained a complete 471bp orf ( open reading frame ) 脱色实验证明沼泽红假单胞菌( rhodopsedomonaspalustris )对偶氮染料有较强的降解能力,我们通过genbank搜索,对所获得的所有偶氮还原酶基因在ncbi进行比对并设计引物,从沼泽红假单胞菌质粒中扩增获得了一条含471bp完整开放阅读框架的序列。 |
| 8. | In this study , iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois . a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ) . the product of pcr was linked with suitable plasmid . then , the recombined plasmid was converted to escherichia coli . the converted escherichia coli 根据已发表的iltvtk基因的核苷酸序列设计一对pcr引物,以增殖的两株iltv的dna为模板,分别对它们的tk基因进行pcr扩增。将回收的pcr产物连接到适当的质粒载体上,转化感受态大肠杆菌,通过筛选对iltvtk基因的阳性克隆进行扩增培养。 |
| 9. | The phylogenic relation and similarity of the gene is full length of lfy - like gene , which is 99 % similar both in nucleic acid sequence and amino acid sequences with that of ginlfy f rom the female . compared with the ginlfy of female , the gene from the male tree is deficient in 3 bp , and mutantion sites exists on the varied region of lfy - like 该基因与文献报道的银杏雌株leafy基因相比,核苷酸序列同源性高达99 ,蛋白质序列同源浙江大学博士学位论文摘要性达99 ,缺少了3个核昔酸,突变均在植物leaf ’ y基因的非保守区内。 |
| 10. | The nucleic acids sequence and amino acids sequence of xynbi were homologously analysed with the sequences coding for xylanases published in genebank . the result indicated that xynb ] was the highest similarity to the gene xyli from sm ptomyces sp . strain s38 , up to 86 % 将所测出的基因xynb1的成熟蛋白部分核苷酸序列与genebank上的木聚糖酶基因序列进行同源比较,最高同源性为86 ,氨基酸序列最高同源性也为86 ,并且与之同源性最高的基因所表达的酶酶学性质与xynb酶学性质有较大差异。 |